Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95±0.08, 6.57±0.08 and 3.55±0.55 x 106 cells/mm3 respectively) compared to control (7.34±0.40) at P<0.05. Also, significant decreases (P<0.05) in the level of the total WBC (White Blood Cell) count, platelet count, PCV (Packed Cell Volume) and Hb (haemoglobin) concentration were observed. The decreases were dose dependent. The MCH (Mean Corpuscular Haemoglobin) and MCHC (Mean Corpuscular Haemoglobin Concentration) except MCV (Mean Corpuscular Volume) significantly decreased in treated group only. The triherbal formulation exhibited significant antioxidant activities showing increased levels of SOD, CAT and Protein content due to activation of the enzyme involve in detoxification of free radicals and decreased in the level of GSH and MDA due to accumulation of peroxides and H2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged.
| Published in | Cell Biology (Volume 9, Issue 1) |
| DOI | 10.11648/j.cb.20210901.12 |
| Page(s) | 7-15 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Nigella Sativa, Carica Papaya, Boswellia Sacra, Antioxidant and Haematology
| [1] | Abdullahi, A. A. (2011). Trends and challenges of traditional medicine in Africa. African Journal of Traditional and Complementary and Alternative Medicine. 8: 115–123. |
| [2] | Agnaniet, H., Makani, T., Akagah, A., Menut C. and J. M. Bessiere (2005). Volatile constituents and antioxidant activity of essential oils from Lippia multiflora Mold growing in Gabon. Flavour Fragrance Journal 20: 34–38. |
| [3] | Ahmad, B., Naeem A. K. and Ghufran, A. (2005). Innamudin Pharmacological Investigation of Cassia sophera, Linn. Var. purpurea, Roxb. Medical Journal of Islamic World Academy of Sciences 15(3): 105–109. |
| [4] | Akinnuga, A. M., Bamidele, O., Ekechi, P. and Adeniyi OS (2011). Effects of an Ethanolic Leaf Extract of Gongronema latifolium on Haematological Some Parameters in Rats. Africa Journal of Biomedical Research 14: 153-156. |
| [5] | Amusa, N. A., Ashaye, O. A., and Oladapo, M. O. (2003). Biodeterioration of the African Star apple (Chrysophyllum albidum) in storage and the effect on its food value. Afriica Journal. Of Biotechnology 2: 56 - 57. |
| [6] | Antai A. B., Ofem, O. E., Ikpi, D. E., Ukafia, S. and Agiang, E. A. (2009). Phytochemistry and some haematological changes following oral administration of ethanolic root extract of Physiological Sciences 24 (1), 79-83. |
| [7] | AshokKumar, T. (2004). Antioxidants: New-generation therapeutic base for treatment of polygenic disorders. Current science 86: 496-504 |
| [8] | Atasayar, S., Gurer-Orhan, H., Gurel, B. and Ozgunes, H. (2009). Preventive effect of aminiguanidine compared to Vitamin C and vitamin Eon cisplatin –induced nephrotoxicity in rats. Experimental Toxicology and Pathology 61: 23-34 |
| [9] | Bandaranayake, W. M. (2006). Qualitycontrol, screening, toxicity, and regulation of herbal Drugs in Modern Phytomedicine. Turning Medicinal Plants into Drugs 6: 25–57. |
| [10] | Chia. S., Nagurney, J. T., Brown, D. F., Raffel, O. C., Bamberg, F., Senatore, F., Wackers, F. J. and Jang, I. K. (2009). Association of leucocyte and neutrophil counts with infarct size, left ventricular function and outcome after percutaneous coronary intervention for ST- elevation myocardial infarction. America Journal of Cardiology 103: 333–337. |
| [11] | Choudhury, S., Sharan, L., and Sinha, M. P. (2015). Screening of Some Commonly Used Medicinal Plants against Enteric Human Pathogen Vibrio cholera. European Journal of Medical Physics 9(3): 1–6. |
| [12] | Deger, S., Deger, Y., Ertekin, A., Gul, k. and Ozdal, N. (2008). Determination of the statusof lipid perioxidation and antioxidant in Cattle infected with Dictyocaulus viviparous. Turkish Parasitology 32: 234-237. |
| [13] | Ekiz, C., Agaoglu, L., Karakas, Z., Gurel, N. and Yelcin, I. (2005). The effect of iron deficiency anemia on the function of immune system. Hematological Journal 5: 579–583. |
| [14] | Ekor, M. (2014). The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology 2: 1–10. |
| [15] | Fabricant, D. S. and Farnsworth, N. R. (2001). The Value of Plants Used in Traditional Medicine for Drug Discovery. Environmental Health Perspectives. 109: 69–75. |
| [16] | Fatima, A., Singh, P. P., Agarwal, P. I. and Raghuveer, A. S. (2013). Treatment of various diseases by Carissa spinarum l. - A promising shrub. International Journal of Pharmaceutical Sciences and Research 4 (7): 2489-2495. |
| [17] | George, J. N. (2000). Platelets. Lancet 355: 1531 – 1539. |
| [18] | Ghaffar, A. S., Ashraf, R., Hussain, T., Hussain, M., Shafique, S. and Aslam, S. (2014). Clinicohematological disparities induced by triazophos (organophosphate) in Japanese quail. Pakistan Veterinary Journal 34: 257-259. |
| [19] | Hassan, R., Acta, A. and Abdul, B. (2012). A Medicinal Plants (Importance and Uses), Pharmaceutica Analytica 3 (10): 41-52. |
| [20] | Hassan, S. A. Ahmed, W. A. Galeb, F. M. El-Taweel, M. A. and Abu-Bedair, F. A. (2008). In vitro challenge using thymoquinone on hepatocellular carcinoma (HepG2) cell line. Iran Journal of Pharmaceutical Research 7(4): 283–290. |
| [21] | Hussain, R., Khan, F., Mahmood, S., Rehan, S. and Ali, F. (2014). Clinicohematological and tissue changes induced by butachlor in male Japanese quail (Coturnix japonica). Pest Biochemistry and Physiology 109: 58- 63. |
| [22] | Jonas, C. R., Puckett, A. B., Jones, D. P., Griffith, D. P., Szeszycki, E. E., Bergman, G. F., Furr, C. E., Tyre, C., Carlson, J. L., Galloway, J. R., Blumberg, J. B. and Ziegler, T. R. (2000) Plasma antioxidant status after high-dose chemotherapy a randomized trial of parenteral nutrition in bone marrow transplantation patients. The American Journal of Clinical Nutrition 72: 181-189. |
| [23] | Kori-Siakpere O. (2011). Alterations in some haematological parameters of the African Snakehead: Parachanna africans exposed to cadmium. Notulae Science and Biology 3: 29-34 |
| [24] | Marcus, C., Karin, L., Jain, G., Matthias, L. D., Jorns, F., Tilman, G. and Wurgen, S. (2003). Captive roe deer (Capreolus capreolus) select for low amount of tannic acid but not quebracho: flunctuation of preference and potential benefit. Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 136 (2): 369 -382. |
| [25] | Maridass, M. and Britto, A. J. (2008). Origins of Plant Derived Medicines. Ethnobotanical Leaflets 12: 373-387. |
| [26] | Omoregie, E. S. and Osagie, A. U. (2007). Phytochemical Screening and anti-anaemia effect of Jatropha tanjoresis leaf in protein malnourished rats. Plant Achieve 7: 509-516 |
| [27] | Omoregie, E. S. and Osagie, A. U. (2011). Effect of Jatropha tanjoresis leaves supplement on activities of some antioxidant enzymes, vitamins and lipid peroxidation in rat. Journal of Food Biochemistry 35(2): 409-424. |
| [28] | Pan, S. Y., Gerhard, L., Si-Hua, G. and Shu, F. Z. (2014). Historical Perspective of Traditional Indigenous Medical Practices: The Current Renaissance and Conservation of Herbal Resources. Evidence-Based Complementary and Alternative Medicine. 2014/525340. |
| [29] | Schneider, C. R., Sheidt, K. and Brietmaier, E. (2003). Four new pregnant glycosides from Gongronema latifolium (Asclepidaceous). Journal Parkische Chem Chenisker-Zutung 353: 532-536. |
| [30] | Shafaquat, N., Syed, T. and Showkat, A. G. (2017). Glutathione-S-transferase, Superoxide Dismutase (GST, SOD) levels, Protein content and lipid Perioxidation in Schizothorax plagiostomus under the infection of pomphorhynchus in Nallah Sukhnag of Kashmir Valley. Pakistan Journal of Biological Sciences 20: 442-446. |
| [31] | Sharaf, S., AKhan, M. Z., Khan, F., Aslam, M. K., Saleemi and Mahmood, F. (2010). Clinico-hematological and micronuclear changes induced by cypermethrin in broiler chicks: their attenuation with vitamin E and selenium. Experimental Toxicology and Pathology 62: 333-341. |
| [32] | Siwela, A. H., Motsi, L. R. and Dube, S. (2013). Alternation of some hepatic enzyme activities by gastrointestinal helminth parasite in domesticated ostrishes. Advance in Bioresearch 4: 145-150. |
| [33] | Sofowora, A., Ogunbodede, E., Onayade, A. (2013). The role and place of medicinal plants in the strategies for disease. African Journals Online 10: 210–229. |
| [34] | Telefo, P. B., Lienou, L. L., Yemele, M. D., Lemfack, M. C., Mouokeu, C., Goka, C. S. and Moundipa, F. P. (2011). Ethnopharmacological survey of plants used for the treatment of female infertility in Baham, Cameroon. Journal of Ethnopharmacology, 136(1): 178–187. |
| [35] | Vinha, A. F., Soares, M. O., Castro, A., Santos, A. and Machado, M. (2011). Phytochemical Characterization and Radical Scavenging Activity of Aqueous Extracts of Medicinal Plants from Portugal 2: 335–347. |
| [36] | World Health Organisation (2013). The WHO Traditional Medicine Strategy 2014–2023. |
| [37] | World Health Organization (2005). National policy on traditional medicine and regulation of herbal medicines. Report of a WHO global survey. |
| [38] | Yadav, Y. C., Srivastav, D. N. and Saini T. (2010). Nephroprotective and curative activity of lepidium stivum L. seeds in albino rat using cisplatin-induced acute renal failure. Journal of Pharmaceutical chemistry 2: 57-64. |
APA Style
Kehinde Sowunmi, Adebayo Sofiyyah Modupeola, Adesiyan Ayobami Lawal, Kade Emmanuel Ayomikun, Gurpreet Kaur. (2021). Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular. Cell Biology, 9(1), 7-15. https://doi.org/10.11648/j.cb.20210901.12
ACS Style
Kehinde Sowunmi; Adebayo Sofiyyah Modupeola; Adesiyan Ayobami Lawal; Kade Emmanuel Ayomikun; Gurpreet Kaur. Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular. Cell Biol. 2021, 9(1), 7-15. doi: 10.11648/j.cb.20210901.12
AMA Style
Kehinde Sowunmi, Adebayo Sofiyyah Modupeola, Adesiyan Ayobami Lawal, Kade Emmanuel Ayomikun, Gurpreet Kaur. Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular. Cell Biol. 2021;9(1):7-15. doi: 10.11648/j.cb.20210901.12
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Download@article{10.11648/j.cb.20210901.12,
author = {Kehinde Sowunmi and Adebayo Sofiyyah Modupeola and Adesiyan Ayobami Lawal and Kade Emmanuel Ayomikun and Gurpreet Kaur},
title = {Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular},
journal = {Cell Biology},
volume = {9},
number = {1},
pages = {7-15},
doi = {10.11648/j.cb.20210901.12},
url = {https://doi.org/10.11648/j.cb.20210901.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20210901.12},
abstract = {Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95±0.08, 6.57±0.08 and 3.55±0.55 x 106 cells/mm3 respectively) compared to control (7.34±0.40) at P2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged.},
year = {2021}
}
TY - JOUR T1 - Evaluation of Biochemical, Hematological and Antioxidant Properties in Mice Exposed to a Triherbal (Nigella sativa, Carica papaya and Boswellia sacra) Formular AU - Kehinde Sowunmi AU - Adebayo Sofiyyah Modupeola AU - Adesiyan Ayobami Lawal AU - Kade Emmanuel Ayomikun AU - Gurpreet Kaur Y1 - 2021/04/29 PY - 2021 N1 - https://doi.org/10.11648/j.cb.20210901.12 DO - 10.11648/j.cb.20210901.12 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 7 EP - 15 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20210901.12 AB - Nigella sativa, Carica papaya and Boswellia sacra are medicinal plants in the commonly used in folkloric medicine due to the presence of its immense therapeutic properties. Fifty (50) female albino mice weighing between 15-22g were divided into five groups of 10 mice each. Animal in group 1 served as control group and were administered distilled water while animal in group 2 were given 2ml of cisplatin (orally). Animal in group 3-5 were given orally; 100 mg/kg (low dose), 200 mg/kg (medium dose) and 400 mg/kg (high dose) of triherbal preparation. The feeding regimens lasted for 28 days. After 28 days, mammary gland and blood samples were collected for haematological and antioxidant analysis. The triherbal formula decreased the GSH and MDA levels of mice treated with 100 mg/kg and 400 mg/kg doses compare to control. The measurement of total protein content, SOD and CAT increased in treated animals compared to control. However, RBC (Red Blood Cell) counts significantly decreased in the low, medium and high dose groups (0.95±0.08, 6.57±0.08 and 3.55±0.55 x 106 cells/mm3 respectively) compared to control (7.34±0.40) at P2O2. Also, decreased in haematological parameters due to the presence of phytochemicals such as phenol, resins, saponins, sterols, tannis and terpenes in the triherbal formula. Therefore, it has potential to induce haematotoxicity hence consumption of high concentrations should be discouraged. VL - 9 IS - 1 ER -
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